Ddx4 (NM_001145885) Mouse Recombinant Protein
CAT#: TP526455
Purified recombinant protein of Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 (Ddx4), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
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CNY 7,106.00
货期*
现货
规格
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经常一起买 (1)
DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
CNY 600.00
Specifications
Product Data | |
Species | Mouse |
Expression Host | HEK293T |
Expression cDNA Clone or AA Sequence |
>MR226455 protein sequence
Red=Cloning site Green=Tags(s) MGDEDWEAEILKPHVSSYVPVFEKDKYSSGANGDTFNRTSASSEMEDGPSGRDDFMRSGFPSGRSLGSRD IGESSKKENTSTTGGFGRGKGFGNRGFLNNKFEEGDSSGFWKESNNDCEDNQTRSRGFSKRGGCQDGNDS EASGPFRRGGRGSFRGCRGGFGLGRPNSESDQDQGTQRGGGLFGSRKPAASDSGNGDTYQSRSGSGRGGY KGLNEEVVTGSGKNSWKSETEGGESSDSQGPKVTYIPPPPPEDEDSIFAHYQTGINFDKYDTILVEVSGH DAPPAILTFEEANLCQTLNNNIAKAGYTKLTPVQKYSIPIVLAGRDLMACAQTGSGKTAAFLLPILAHMM RDGITASRFKELQEPECIIVAPTRELINQIYLEARKFSFGTCVRAVVIYGGTQFGHSVRQIVQGCNILCA TPGRLMDIIGKEKIGLKQVKYLVLDEADRMLDMGFGPEMKKLISCPGMPSKEQRQTLLFSATFPEEIQRL AGDFLKSSYLFVAVGQVGGACRDVQQTILQVGQYSKREKLVEILRNIGDERTMVFVETKKKADFIATFLC QEKISTTSIHGDREQREREQALGDFRCGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRT GRCGNTGRAISFFDTDSDNHLAQPLVKVLSDAQQDVPAWLEEIAFSTYVPPSFSSSTRGGAVFASVDTRK NYQGKHTLNTAGISSSQAPNPVDDESWD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Tag | C-MYC/DDK |
Predicted MW | 79.3 kDa |
Concentration | >0.05 µg/µL as determined by microplate BCA method |
Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
Storage | Store at -80°C after receiving vials. |
Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
Reference Data | |
RefSeq | NP_001139357 |
Locus ID | 13206 |
UniProt ID | Q3V086 |
Refseq Size | 2850 |
Cytogenetics | 13 63.87 cM |
Refseq ORF | 2187 |
Synonyms | AV206478; Mvh; VASA |
Summary | ATP-dependent RNA helicase required during spermatogenesis to repress transposable elements and preventing their mobilization, which is essential for the germline integrity (PubMed:20439430, PubMed:28633017). Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons (PubMed:20439430, PubMed:28633017). Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle (PubMed:20439430, PubMed:28633017). Required for PIWIL2 slicing-triggered piRNA biogenesis: helicase activity enables utilization of one of the slice cleavage fragments generated by PIWIL2 and processing these pre-piRNAs into piRNAs (PubMed:28633017).[UniProtKB/Swiss-Prot Function] |
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