RUNX1T1 Human shRNA Plasmid Kit (Locus ID 862)

Specifications

Product Data
Product Name	RUNX1T1 Human shRNA Plasmid Kit (Locus ID 862)
Locus ID	862
UniProt ID	Q06455
Synonyms	AML1-MTG8; AML1T1; CBFA2T1; CDR; ETO; MTG8; ZMYND2
Vector	pGFP-C-shLenti
Format	Lentiviral plasmids
Kit Components	RUNX1T1 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 862). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq	NM_001198625, NM_001198626, NM_001198627, NM_001198628, NM_001198629, NM_001198630, NM_001198631, NM_001198632, NM_001198633, NM_001198634, NM_001198679, NM_004349, NM_175634, NM_175635, NM_175636, NM_175636.1, NM_175636.2, NM_004349.1, NM_004349.2, NM_004349.3, NM_175634.1, NM_175634.2, NM_175635.1, NM_175635.2, NM_001198633.1, NM_001198634.1, NM_001198625.1, NM_001198632.1, NM_001198626.1, NM_001198627.1, NM_001198628.1, NM_001198629.1, NM_001198630.1, NM_001198631.1, BC005850, BC067078, BC139783, NM_001198625.2, NM_175634.3, NM_175635.3, NM_001198629.2, NM_001198630.2, NM_001198634.2, NM_004349.4, NM_001198627.2, NM_001198628.2, NM_001198631.2
Summary	This gene encodes a member of the myeloid translocation gene family which interact with DNA-bound transcription factors and recruit a range of corepressors to facilitate transcriptional repression. The t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities in acute myeloid leukemia. The translocation produces a chimeric gene made up of the 5'-region of the runt-related transcription factor 1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.