Gp2 Mouse shRNA Plasmid (Locus ID 67133)

Specifications

Product Data
Product Name	Gp2 Mouse shRNA Plasmid (Locus ID 67133)
Locus ID	67133
UniProt ID	Q9D733
Synonyms	2310037I18Rik; AV060639
Vector	pRS
Format	Retroviral plasmids
Kit Components	Gp2 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 67133). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	BC119573, NM_025989, NM_025989.1, NM_025989.2, NM_025989.3
Summary	Functions as an intestinal M-cells transcytotic receptor specific of type-I-piliated bacteria that participates to the mucosal immune response toward these bacteria. At the apical membrane of M-cells binds fimH, a protein of the bacteria type I pilus tip. Internalizes bound bacteria, like E.coli and S.typhimurium, from the lumen of the intestine and delivers them, through M-cells, to the underlying organized lymphoid follicles where they are captured by antigen-presenting dendritic cells to ellicit a mucosal immune response.[UniProtKB/Swiss-Prot Function]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.