Mycobacterium tuberculosis (38 kDa Ag) Mouse Monoclonal Antibody [Clone ID: A2H8]

Specifications

Product Data
Clone Name	A2H8
Applications	ELISA, WB
Recommend Dilution	ELISA: Reactive to the immunogen in ELISA assay.  The monoclonal antibody reacts with the recombinant M. tuberculosis (TB) protein in ELISA assay. Western Blotting: The monoclonal antibody detects the recombinant M. tuberculosis (TB) protein on Western blot. A band corresponding to the immunogen is detected.
Reactivity	Mycobacterium tuberculosis
Host	Mouse
Clonality	Monoclonal
Immunogen	A recombinant M. tuberculosis protein cloned from virulent strain H37Rv with primers targeting the 38Kd antigen.
Specificity	This M. tuberculosis antibody is reactive with the recombinant immunogen (M. tuberculosis 38Kd antigen).  Reactivity with the native 38KD antigen in TB H37Rv has not been tested.
Formulation	0.01M PBS, pH 7.0 without preservatives State: Aff - Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore with Double distillated water to adjust the final concentration to 1.0 mg/ml.
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Background	Mycobacterium tuberculosis is the most common cause of tuberculosis. Primary infection begins with inhalation of 1 to 10 aerosolised bacilli. The pathogenicity of the organism is determined by its ability to escape host immune responses as well as eliciting delayed hypersensitivity. Alveolar macrophages engulf the invading cells but are unable to mount an effective defense. Several virulence factors are responsible for this apparent failure; most notably in the mycobacterial cell wall are the cord factor, lipoarabinomannan, and the 65 kd heat shock protein or HSP65. The emergence of new strains of resistant Mycobacterium tuberculosis has created new interest in clinical diagnosis. Studies have shown immunohistochemical techniques to be superior to conventional special stains. Thus the demonstration of mycobacterial antigens are not only useful in establishing mycobacterial aetiology, but can also be used as an alternative method to the conventional Ziehl-Neelsen method.
Synonyms	M. tuberculosis, TB
Reference Data

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