Glypican 3 (GPC3) Mouse Monoclonal Antibody [Clone ID: 1G12]

Specifications

Product Data
Clone Name	1G12
Applications	IHC
Recommend Dilution	Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/50-1/150 Pretreatment of deperaffinized tissue with heat-induced epitope retrieval is recommended. Use Polymer anti Mouse/Rabbit IgG as a detection system. Positive Control: Hepatocellular carcinoma.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	A fragment containing the last 70 amino acids of the core glypican-3.
Specificity	The 1G12 monoclonal antibody has been used to assess GPC3 expression in malignant and nonmalignant liver tissue samples by Immunohistochemistry in formalin-faxed and paraffin-embedded tissue. Cellular Localization: Cytoplasmic and membrane.
Formulation	State: Supernatant State: Tissue Culture Supernatant with 0.2% BSA and 15mM Sodium Azide.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	glypican 3
Database Link	Entrez Gene 3958 HumanEntrez Gene 2719 Human UniProt ID P51654
Background	Glypican-3 (GPC3) is a member of the glypican family of glycosyl phosphatidylinositol-anchored cell-surface heparan sulfate proteoglycans. Studies have shown that GPC3 is expressed at the protein level in most hepatocellular carcinomas, but it is undetectable in normal liver and benign hepatic lesions, including dysplastic and cirrhotic nodules. In addition, GPC3 is significantly elevated in the serum of a large proportion of patients with hepatocellular carcinoma. Based on these results, it has been proposed that GPC3 could be a useful marker to differentiate between benign and malignant liver diseases.
Synonyms	Intestinal protein OCI-5, GTR2-2, MXR7, OCI5
Reference Data
Protein Families	Secreted Protein

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