Asgr1 Mouse Monoclonal Antibody [Clone ID: 8D7]

Specifications

Product Data
Clone Name	8D7
Applications	ELISA, IF, IHC, WB
Recommend Dilution	Immunohistochemistry on Frozen Sections (Ref.1-3,5): Aceton fixed tissue section were incubated Hybridoma culture supernatant. Flow Cytometry (Ref.4). Immunoassay (Ref.1,3): Microtiter plates were coated with 100µl 20 µg/ml for ELISA. Immunflourescence (Ref.7). Western blot (Ref.2,4,6): non-reduced sample treatment and SDS-Page was used. The band size is 42 kDa (4); 10% SDS-PAGE followed by blotting on nitrocellulose or PDFM, block with 10% non-fat dry milk. Positive Control: Rat hepatocytes.
Reactivity	Human, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Crude Rat Liver membrane extracts.
Specificity	This antibody recognizes a subunit-specific epitope on RHL-1 of rat ASGPR.
Formulation	PBSState: Purified State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 0.1% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Protein G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	asialoglycoprotein receptor 1
Database Link	Entrez Gene 24210 Rat UniProt ID P02706
Background	The asialoglycoprotein (ASGP) receptor is a transmembrane hepatocellular surface carbohydrate binding glycoproteins lacking terminal sialic acid residues (asialoglycoproteins). Characterization of the ASGP receptor  revealed its functional role in the binding, internalization and transport of a wide range of glycoproteins, which have exposed galactose or N-acetylgalactosamine residues, via the process of receptor-mediated endocytosis (RME). The ASGP receptor can bind a variety of important plasma proteins including transport proteins (i.e. transferrin), enzymes such as alkaline phosphatase, immunoglobulins including IgA, apoptotic hepatocytes, fibronectin and platelets. Additionally, the expression of the ASGP receptor has been clinically correlated to the level of hepatic function that is lost during liver diseases related to cancer, viral hepatitis, and cirrhosis. The ASGP receptor consists of major and minor subunits, which in the rat were identified as rat hepatic lectin (RHL) 1 and RHL 2/3, with molecular weights of respectively 42, 49 and 54 kDa. The selective binding (calcium and pH depended) and uptake of terminal galactosyl bearing proteins requires the formation of hetero-oligomers between these major and minor forms The total ASGP receptor population consisted of two functionally distinct receptor populations, designated State 1 and State 2, which were involved in the endocytosis and intracellular processing of ligands by different pathways.
Synonyms	ASGP-R 1, ASGPR 1, Asialoglycoprotein receptor 1, Hepatic lectin H1
Reference Data

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