TNFRSF1A Mouse Monoclonal Antibody [Clone ID: MR1-2]

Specifications

Product Data
Clone Name	MR1-2
Applications	ELISA, IHC, IP, WB
Recommend Dilution	Cell Culture Experiments.  Flow Cytometry: Use 1/10 as starting dilution.  Advised Positive Control: PHA activated T cells. Immuno Assays. Western blotting. Immunoprecipitation. Immunohistochemistry on Frozen Sections: Use 1/10 as starting dilution.  Advised Positive Control: Human Lymphnodes.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Specificity	The antibody reacts with the extra-cellular part of the TNF-R I. It also reacts with the soluble receptor. TNF-R I is present on most cell types and is considered to play a prominent role in cell stimulation by TNF-alpha: Induction of cytotoxicity and other functions are mediated largely via TNF-R I. The antibody cross reacts with Rhesus and Cynomolgus natural TNF-R I. The antibody is an agonistic antibody. The reactivity of the antibody with cell-bound TNF-Receptor is minimally inhibited by high concentrations of TNF-alpha.
Formulation	PBS State: Purified State: Liquid (0.2 μm filtered) Ig fraction Stabilizer: 0.1% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	tumor necrosis factor receptor superfamily member 1A
Database Link	Entrez Gene 7132 Human UniProt ID P19438
Background	Tumor Necrosis Factor (TNF) is a cytokine whose function is mediated through two distinct cell surface receptors (TNF Receptor I and TNF Receptor II) that are included in the TNF Receptor superfamily along with FAS antigen and CD40. TNF Receptors I and II are 55 and 75 kDa members, respectively, of a family of cell surface molecules including nerve growth factor receptor, Fas/Apo1, CD30, OX40, and 41BB, which are characterized by cysteine rich motifs in the extracellular domain. While TNF Receptor I and TNF Receptor II share 28% sequence homology in the extracellular domains, their intracellular domains lack sequence homology, suggesting that they differ in their internal signal transduction pathways. TNF Receptor I contains an approximately 80 amino acid death domain near its carboxy terminus capable of transmitting an apoptotic signal through its interaction with TRADD (TNF Receptor I associated death domain protein), and subsequent interactions with FADD. TNF Receptor I can also activate the transcription factor NFkB via TRAF2 (TNF Receptor associated factor 2). The cytoplasmic domain of TNF Receptor I can directly interact with Jak kinase, thereby activating the JAK/STAT signal transduction cascade. TNF Receptor I is expressed by virtually all nucleated mammalian cells, including hepatocytes, monocytes and neutrophils, cardiac muscle cells, endothelial cells, and CD34 + hematopoietic progenitors. Both TNF alpha and TNF beta bind to TNF Receptor I.
Synonyms	Tumor necrosis factor receptor 1, TNF-R1, TNF-RI, TNFR-I, p55, p60, Tnfrsf1a
Reference Data

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