Interferon alpha 2 (IFNA2) Mouse Monoclonal Antibody [Clone ID: N39]

Specifications

Product Data
Clone Name	N39
Applications	ELISA, FN, Neutralize, WB
Recommend Dilution	ELISA. Western Blot. Immunhistochemistry on paraffin sections.  N39 is one of many MoAbs described by Kontsek et al. (1991) directed against one of two immunodominant epitopes of IFNα(II), called site I. Its counterpart MoAb is N27 directed against a third "atypical" site. Site I is formed by the carboxyterminal domain residues 112-148.  This site is responsible for the antiviral and antiproliferative activities. N39 and its counterpart N27 detecting different epitopes on IFNα(II) represent a good combination of antibodies to set up an ELISA assay for quantisation of interferon, e.g. after viral infections.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	A BALB/c mouse was immunized with E-coli derived, purified recombinant IFN-alpha 2c and spleen cells were fused with mouse myeloma cells NS1.
Specificity	This antibody reacts to IFN-alpha (II).
Formulation	PBS State: Purified State: Liquid purified Ig fraction Preservative: 0.02% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	interferon, alpha 2
Database Link	Entrez Gene 3440 Human UniProt ID P01563
Background	The alpha interferons are involved in virus resistance in target cells for these viruses. They are known to block cell proliferation and to regulate MHC class I antigen expression. The IFNalppha family has over .. genes and pseudogenes in two families (I and II), one with a mature length of 166aa and one of 172aa. Cells producing IFNalpha are lymphocytes, monocytes, macrophages and cell lines such as Namalwa and KGI. Bioassays for IFNalpha include cytopathic effect blocking, by viruses such as VSV, SFV and BMCV, on their target cells. A number of receptors for IFNalpha are now known and seem to be expressed on most cell types. N39 is specific for INFalpha(II) and does not crossreact with INFalpha(I).
Synonyms	Interferon alpha-A, LeIF A, IFN-alpha 2
Reference Data

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