MUC2 Mouse Monoclonal Antibody [Clone ID: CCP58]

Specifications

Product Data
Clone Name	CCP58
Applications	ELISA, FC, IF, IHC, IP, WB
Recommend Dilution	ELISA: Use Antibody without BSA For coating. Western blot: 0.5-1 µg/ml. Immunoprecipitation:  1-2 µg/500 µg protein lysate.  Flow Cytometry: 0.5-1 µg/106 cells.  Immunofluorescence: 1-2 µg/ml. Immunohistochemistry on Frozen and Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris-HCl buffer, pH 10.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: Colon cancer cell line LS174T, Small intestine.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	A synthetic peptide of 29 amino acids, from Human MUC2 protein (MI-29) (VNTR region), coupled to KLH.
Specificity	This Monoclonal CCP58 antibody Recognizes a single glycoprotein of 520kDa, identified as mucin 2 (MUC2) and shows no cross-reaction with Human milk fat globule membranes, MUC1, or MUC3.  Clone CCP58 is reported to react with colon gastric cancer cells, normal intestine, colon and salivary gland (immunoperoxidase staining), Human colon cancer cell line by Flow Cytometry and Western blotting.  The CCP58 antibody is known to be specific to amino acids GTQTP (GlyThrGlnThrPro). Cellular Localization: Cytoplasmic and cell surface.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Predicted Protein Size	520 kDa
Gene Name	mucin 2, oligomeric mucus/gel-forming
Database Link	Entrez Gene 4583 Human UniProt ID Q02817
Background	This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by this gene is secreted and forms an insoluble mucous barrier that protects the gut lumen. The protein polymerizes into a gel of which 80% is composed of oligosaccharide side chains by weight. The protein features a central domain containing tandem repeats rich in threonine and proline that varies between 50 and 115 copies in different individuals. Alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.
Synonyms	MUC-2, MUC2, SMUC, Intestinal mucin-2
Reference Data

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