Lep Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	ELISA: Direct: To detect Rat Leptin by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 - 1.0 μg/ml is required. In conjunction with compatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat Leptin. Sandwich: To detect Rat Leptin by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 - 1.0 μg/ml is required. In conjunction with Polyclonal Anti-Rat Leptin as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat Leptin. Western Blot: To detect Rat Leptin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat Leptin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Highly pure (> 98 %) recombinant Rat Leptin
Specificity	This antibody detects Leptin.
Formulation	PBS, pH 7.2 Label: Biotin State: Sterile filtered lyophilized Ig fraction
Reconstitution Method	Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/ml.
Purification	Affinity chromatography
Conjugation	Biotin
Storage Condition	Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C. Avoid repeated freezing and thawing.
Gene Name	leptin
Database Link	Entrez Gene 25608 Rat UniProt ID P50596
Background	Leptin plays a critical role in the regulation of body weight by inhibiting food intake and stimulating energy expenditure. Defects in Leptin production cause severe hereditary obesity in rodents and humans. In addition to its effects on body weight, leptin has a variety of other functions, including the regulation of hematopoiesis, angiogenesis, wound healing, and the immune and inflammatory response. The Leptin gene is the human homolog of the gene (ob) mutant in the mouse 'obese' phenotype. Defects in the Leptin gene are the cause of profound obesity and type II diabetes.
Synonyms	LEP, OB, OBS, Obesity factor, Obese protein
Reference Data

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