Bone Sialoprotein (IBSP) Chicken Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FC, IF, IHC, R, WB
Recommend Dilution	ELISA: 1 µg/ml. RIA: 20 ng/ml (Ref.1,4). Flow Cytometry (Ref.2). Western blot: 1/100 (Ref.1,3). Immunohistochemistry on Paraffin Sections: 4 µg/ml (Ref.4).
Reactivity	Human
Host	Chicken
Clonality	Polyclonal
Immunogen	Human Bone Sialoprotein (bone extract)
Specificity	This antibody detects Bone Sialoprotein. There were no cross reactivities obtained with Human Osteonectin, Human Osteopontin and Human Alkaline Phosphatase.
Formulation	PBS, pH 7.4 without preservatives. State: Purified State: Lyophilized purified IgY fraction.
Reconstitution Method	Restore in aqua bidest to initial volume.
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	integrin binding sialoprotein
Database Link	Entrez Gene 3381 Human UniProt ID P21815
Background	The protein encoded by the Bone Sialoprotein gene is a major structural protein of the bone matrix. It is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts, and it constitutes approximately 12% of the noncollagenous proteins in human bone. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor.
Synonyms	Bone sialoprotein II, BSP II, IBSP, BNSP
Note	LocusID 3381
Reference Data

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