BubR1 (BUB1B) (N-term) Sheep Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western Blot: 1 - 10 µg/ml; Detects a 130 kDa band. Immunohistochemistry.
Reactivity	Mouse
Host	Sheep
Clonality	Polyclonal
Immunogen	Recombinant protein corresponding to amino acids 10 - 190 of the mouse Bub1b protein.
Specificity	This antibody reacts to Bub1b (N-Term).
Formulation	Phosphate buffered saline with 0.08% sodium azide State: Purified State: Liquid purified Ig
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	The antibody can be shipped at ambient temperature. Store (in aliquots) at -20°C only. Avoid repeated freezing and thawing.
Gene Name	BUB1 mitotic checkpoint serine/threonine kinase B
Database Link	UniProt ID O60566
Background	Bub1, a mitotic checkpoint gene first identified in yeast, ensures proper chromosome segregation by delaying anaphase until chromosomes are properly aligned on the mitotic spindle. Mutations in the mitotic spindle assembly checkpoint genes, like yeast Bub1, cause cells to exit mitosis without proper chromosome attachment/alignment, resulting in aneuploidy. Aneuploidy is a form of genetic instability involved in cancer. Recently, a novel homologue of the murine Bub1 gene has been identified and named Bub1b. The yeast mitotic checkpoint pathway is unresolved, but the mammalian pathway remains even less clear. It is important to investigate the role of the Bub1 genes in the mammalian checkpoint as they have been implicated in carcinogenesis, namely colon carcinomas. The aneuploidy and chromosome instability characterizing some tumors is thought to be caused by mutations in Bub1.
Synonyms	Protein SSK1, BUBR1, MAD3L, BUB1 beta
Reference Data

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