APAF1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western blot: 1/1000-1/2000.  Immunohistochemistry on Paraffin Sections: Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide corresponding to amino acids 12-28 of Human Apaf1
Specificity	This antibody recognises a 130kD band in Western blots.
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Afinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	apoptotic peptidase activating factor 1
Database Link	Entrez Gene 317 Human UniProt ID O14727
Background	Apaf1 (Apoptosis protease activating factor 1) is the mammalian homologue of the CED-4 cell death protein in C. Elegans. Apaf1 binds to cytochrome C and caspase-9 leading to caspase-9 activation, resulting in the beginning of the proteolytic cascade characteristic of apoptosis. Apaf1 can also associate with caspase-4 and caspase-8.
Synonyms	Apaf-1, KIAA0413
Reference Data

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