Alkaline phosphatase / ALPI / IAP Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	ELISA: 1/4,000-1/20,000 Western Blot: 1/500-1/2,000.
Reactivity	Bovine
Host	Goat
Clonality	Polyclonal
Immunogen	Alkaline Phosphatase [Calf intestine].
Specificity	Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Alkaline Phosphatase [Calf intestine]. Cross reactivity against Alkaline Phosphatase from other tissues and species may occur but have not been specifically determined. No reaction was observed against the bacterial form of the enzyme.
Formulation	0.01M Sodium Phosphate, 0.14M Sodium Chloride, pH 7.2 State: Serum State: Lyophilized Serum prepared from by a Delipidation and Defibrination.
Reconstitution Method	Restore with 1.0 ml of deionized water (or equivalent).
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	UniProt ID P19111
Background	Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.
Synonyms	Intestinal alkaline phosphatase
Reference Data

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