Horseradish Peroxidase / HRP Mouse Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and most immunological methods requiring high titer and specificity. Dot Blot.  Western Blot: 1/500-1/2,000. ELISA: 1/5,000-1/20,000. Immunoprecipitation.
Host	Mouse
Clonality	Polyclonal
Immunogen	Native Peroxidase [Horseradish]
Specificity	This Peroxidase antibody was prepared from monospecific antiserum by a Delipidation and Defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Peroxidase [Horseradish].  Cross reactivity against Peroxidase from other tissues and species may occur but have not been specifically determined.
Formulation	0.02M Sodium Phosphate, 0.15M Sodium Chloride, pH 7.2 State: Serum State: Lyophilized Serum Stabilizer: None Preservative: 0.01% (w/v) Sodium Azide
Reconstitution Method	Restore with 2.0 ml of deionized water (or equivalent).
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Background	Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
Reference Data

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