lacI Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	ELISA: 1/90,000–1/100,000. Western Blot: 1/500-1/2,000. Expect a band approximately ~38 kDa in size corresponding to Lac I by Western blotting in the appropriate cell lysate or extract.
Reactivity	Escherichia coli
Host	Rabbit
Clonality	Polyclonal
Immunogen	Full-length recombinant Lac I protein
Specificity	This antibody reacts with recombinant Lac I protein. A BLAST analysis was used to suggest cross-reactivity with Lac I from DNA-binding transcriptional repressor in Escherichia coli (strain DH10B) and transcriptional repressor of the lac operon in Shigella sonnei (strain Ss046) based on a 100% homology with the immunizing sequence.
Formulation	0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 containing 0.01% (w/v) Sodium Azide as preservative State: Aff - Purified State: Liquid (sterile filtred) purified Ig
Concentration	lot specific
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. Storage Conditions for Trial Size: This vial contains a relatively low volume of reagent (25 µl). To minimize loss of volume dilute 1:10 by adding 225 µl of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
Database Link	UniProt ID P03023
Background	The lac repressor protein (Lac I) is an important genetic control protein. It controls the expression of the lactose metabolizing enzymes in Escherichia coli. This regulation involves specific recognition of the operator DNA sequence (Lac O) and consequent inhibition of transcription initiation by RNA polymerase; modulation of binding at this target site by small sugar molecules, inducers, provides the basis for regulation. Different ligand binding activities are associated with the isolated domains of Lac I: specific and nonspecific DNA binding with the NH2 termini, and inducer and specific DNA binding with the core protein. This antibody could possibly be used to recover and purify protein/nucleic acid complexes (host and viral proteins associated with the viral DNA) from infected cells.
Synonyms	Lactose operon repressor, b0345, JW0336
Reference Data

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