CYP7B1 (C-term) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC
Recommend Dilution	Peptide ELISA: Detection Limit: 1/128000. Western blot: Preliminary experiments in Human Liver, Kidney and Prostate lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). Immunohistochemistry on Paraffin Sections: 3-6 µg/ml.  In Paraffin Embedded Human Breast shows textured Cytoplasm staining in ductal epithelial cells.
Reactivity	Bovine, Human
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence C-YPDSDVLFRYKVKS, from the C Terminus of the protein sequence according to NP_004811.1.
Specificity	This antibody recognizes CYP7B1.
Formulation	Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified IgG fraction. Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	cytochrome P450 family 7 subfamily B member 1
Database Link	Entrez Gene 9420 Human UniProt ID O75881
Background	P450 enzymes constitute a family of monooxygenase enzymes that are involved in the metabolism of a wide array of endogenous and xenobiotic compounds including cholesterol. CYP8B1 moderates the ratio of cholic acid over chenodeoxycholic acid to control the solubility of cholesterol. P450 cholesterol 7-hydroxylase, CYP7A1, is the rate limiting enzyme of bile acid synthesis in the liver, and its expression is mediated by the bile acid receptor FXR. CYP27A1 catalyzes vitamin D3 25-hydroxylation and is localized to the mitochondria in kidney and liver. CYP7B1 (oxysterol 7-α-hydroxylase) functions as an enzyme in the alternate bile acid synthesis pathway. Specifically, CYP7B1 metabolizes 25-and 27-hydroxycholesterol. The gene encoding human CYP7B1 maps to chromosome 8q21.3. Mutations in the CYP7B1 gene may cause a metabolic defect in bile acid synthesis characterized by elevated urinary bile acid excretion, severe cholestasis, cirrhosis and liver synthetic failure.
Synonyms	Cytochrome P450 7B1
Reference Data
Protein Families	Druggable Genome, P450, Transmembrane
Protein Pathways	Primary bile acid biosynthesis

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