ZWF1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, ID, IF, IP, R, WB
Recommend Dilution	This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of choice. Recommended Working Dilutions: Non-precipitating antibody-binding techniques: 1/10,000-1/70,000.
Reactivity	Bakers Yeast
Host	Rabbit
Clonality	Polyclonal
Immunogen	Glucose-6-Phosphate Dehydrogenase isolated and purified from Baker’s Yeast. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity	This Affinity purified antibody recognizes Glucose-6-Phosphate Dehydrogenase from Baker’s Yeast. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.
Formulation	PBS, pH 7.2 stabilized with Dextran without preservatives and foreign proteins State: Aff - Purified State: Lyophilized purified hyperimmune IgG fraction
Reconstitution Method	Restore by adding 0.5 ml of sterile distilled water
Concentration	lot specific
Purification	Solid Phase Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Database Link	UniProt ID P11412
Background	Glucose 6 Phosphate Dehydrogenase (G6PD) produces pentose sugars for nucleic acid synthesis and is the main producer of NADPH reducing power. Catalytic activity: D glucose 6 phosphate + NADP(+) → D glucono 1,5 lactone 6 phosphate + NADPH. Defects in G6PD are the cause of chronic non spherocytic haemolytic anemia (CNSHA). G6PD deficiency is the most common human enzyme deficiency; one benefit of having G6PD deficiency is that it confers a resistance to malaria.
Synonyms	Glucose-6-phosphate 1-dehydrogenase, Glucose-6-P-Dehydrogenase
Reference Data

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