CRISP2 (81-93) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	Peptide ELISA: Detection Limit: 1/8000. Western blot:  In transfected HEK293 transiently expressing Human CRISP2 (MYC and DYKDDDDK tagged), a band of approx. 35kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration: 0.5-1µg/ml. Primary incubations were for 1 hour. Preliminary experiments gave an approx 55kDa band in Human Testis lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 27.3kDa according to NP_003287.1. The 55kDa band was successfully blocked by incubation with the immunizing peptide. Immunohistochemistry on Paraffin Sections: 3.75 µg/ml (Human Testis).
Reactivity	Canine, Human, Porcine
Host	Goat
Clonality	Polyclonal
Immunogen	Synthetic peptide corresponding to internal sequence amino acids 81-93 of Human CRISP2 (NP_003287.1).
Specificity	Recognizes CRISP2.
Formulation	Tris saline, pH 7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer. State: Aff - Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	cysteine rich secretory protein 2
Database Link	Entrez Gene 7180 Human UniProt ID P16562
Background	Cysteine-rich secretory protein 2 (CRISP2) may regulate some ion channels' activity and therebye regulate calcium fluxes during sperm capacitation. CRISP2 is specific to testis and epididymis.
Synonyms	Cysteine-rich secretory protein 2, CRISP-2, Testis-specific protein TPX-1, Cancer/testis antigen 36, GAPDL5, TPX1, CT36
Reference Data
Protein Families	Secreted Protein

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