GAPDH Sheep Polyclonal Antibody

CAT#: AP21308AF-N

GAPDH sheep polyclonal antibody, Azide Free



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CNY 3,402.00


货期*
5周

规格
    • 10 mg

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Specifications

Product Data
Applications ELISA, ID, IF, IP, R, WB
Recommend Dilution This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of
the customer’s own choice.
Working dilutions in non-precipitating antibody-binding techniques: 1/10-1/300.
Reactivity Rabbit
Host Sheep
Clonality Polyclonal
Immunogen Glyceraldehyde-3-Phosphate Dehydrogenase isolated and purified from Rabbit muscle.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity The antibody recognizes GAPDH from Rabbit muscle.
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
Cross-reactivities against enzymes of other sources may occur but have not been determined.
Formulation PBS, pH 7.2 without preservatives and foreign proteins
State: Azide Free
State: Lyophilized hyperimmune IgG fraction
Reconstitution Method Restore by adding 1.0 ml of sterile distilled water
Concentration lot specific
Purification Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Conjugation Unconjugated
Storage Condition Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Background Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. Besides its functioning as a glycolytic enzyme in cytoplasm, recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of findings appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age-related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is constitutively expressed in almost all tissues at high levels, therefore becoming the marker of choice when a loading control in Western blotting is required. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
Synonyms GAPD, CDABP0047
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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