VCAM1 Rabbit Polyclonal Antibody

CAT#: AP22317PU-N

VCAM1 rabbit polyclonal antibody, Aff - Purified

Size: 50 ug 100 ug

Conjugation: Unconjugated Biotin



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CNY 4,790.00


货期*
5周

规格
    • 100 ug

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Specifications

Product Data
Applications ELISA, WB
Recommend Dilution Sandwich ELISA: To detect Human VCAM-1 by Sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human VCAM-1 (AP22317BT-N or AP22317BT-S) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human VCAM-1.
Western Blot: To detect Human VCAM-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVCAM-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity Human
Host Rabbit
Clonality Polyclonal
Immunogen Highly purified recombinant Human VCAM-1
Specificity Recognizes Human VCAM-1.
Formulation PBS, pH 7.2 without preservatives.
State: Aff - Purified
State: Lyophilized (sterile filtered) purified Ig fraction.
Purification Affinity chromatography employing immobilized Human VCAM-1 matrix.
Conjugation Unconjugated
Storage Condition Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer.
Avoid repeated freezing and thawing.
Gene Name vascular cell adhesion molecule 1
Background CD106 (also known as vascular cell adhesion molecule-1 (VCAM-1) and INCAM-110) is a member of the Ig superfamily of adhesion molecules and is expressed at high levels on cytokine stimulated vascular endothelial cells, and at minimal levels on unstimulated endothelial cells. It is also present on follicular and interfollicular dendritic cells of lymph nodes, myoblasts, and some macrophages. CD106 serves as a ligand for leukocyte integrin alphaBeta (VLA-4 or CD49d/CD29) and mediates cell adhesion of leukocytes to activated endothelium. It plays a role in tumor metastasis.
Synonyms V-CAM 1, INCAM-100, L1CAM, VCAM-1
Reference Data
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
Protein Pathways Cell adhesion molecules (CAMs), Leukocyte transendothelial migration
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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