GLUD1 (+Glutamate dehydrogenase 2) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC, WB
Recommend Dilution	Peptide ELISA: 1/32000 (Detection Limit).  Western blot:  0.3-1 µg/ml. Approx 55kDa band observed in Human Brain (Cerebellum) and Mouse Brain lysates and in Human, Mouse and Rat Liver lysates (calculated MW of 61.4kDa according to Human NP_005262.1, of 61.3kDa according to Mouse NP_032159.1 and of 61.4kDa according to Rat NP_036702.1). Immunofluorescence: Customer found particulate cytoplasm staining in HeLa.  Immunohistochemistry on Paraffin Sections:  3-5 µg/ml. In paraffin embedded Human Cerebral Cortex shows mitochondrial staining in neuronal cell bodies and axons.
Reactivity	Bovine, Canine, Human, Mouse, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the internal region of the protein sequence according to NP_005262.1; NP_036216.2.
Specificity	This antibody recognizes Glutamate Dehydrogenase. This antibody is expected to recognize both reported isoforms (NP_005262.1; NP_036216.2).
Formulation	Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified Ig fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	glutamate dehydrogenase 1
Database Link	Entrez Gene 14661 MouseEntrez Gene 24399 RatEntrez Gene 2746 Human UniProt ID P00367
Synonyms	GLUD1, GLUD, GDH1
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, D-Glutamine and D-glutamate metabolism, Metabolic pathways, Nitrogen metabolism

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