Macrophage Inflammatory Protein 3 alpha (CCL20) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC
Recommend Dilution	Peptide ELISA: 1/16000 (Detection Limit). Western Blot: Preliminary experiments in Human Peripheral Blood Mononucleocytes lysates gave no specific signal but low background (at antibody concentration up to 2µg/ml).  Immunohistochemistry on Paraffin Sections: 6-8 µg/ml. In Paraffin Embedded Human Liver shows local staining between the hepatocytes.
Reactivity	Bovine, Human, Porcine
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the internal region of Human CCL20 (NP_004582.1; NP_001123518.1).  Percent identity by BLAST analysis: Human, Gorilla (100%); Gibbon, Monkey (92%).
Specificity	This antibody is expected to recognize both isoforms (NP_004582.1 and NP_001123518.1).
Formulation	Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified Ig fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	C-C motif chemokine ligand 20
Database Link	Entrez Gene 6364 Human UniProt ID P78556
Background	MIP-3a (also known as C-C motif chemokine 20, small-inducible cytokine A20, macrophage inflammatory protein 3 alpha, MIP-3-alpha, liver and activation-regulated chemokine, CC chemokine LARC and beta chemokine exodus-1) is a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. MIP-3a inhibits proliferation of myeloid progenitors in colony formation assays and may be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. MIP-3a also possesses antibacterial activity against E.coli and S.aureus. MIP-3a is a secreted protein that is expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels of expression are also seen in thymus, prostate, testis, small intestine and colon. C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
Synonyms	CCL20, LARC, MIP3A, MIP-3 alpha, C-C motif chemokine 20, Small-inducible cytokine A20, SCYA20, Exodus-1, Dendritic Cell Marker, DC marker
Reference Data
Protein Families	Druggable Genome, Secreted Protein
Protein Pathways	Chemokine signaling pathway, Cytokine-cytokine receptor interaction

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