LYZ Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC, WB
Recommend Dilution	In enzyme-immunocytochemical and immunohistochemical techniques for the detection of lysozyme at the cellular and subcellular level in appropriately treated cell and tissue substrates; as detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA, Western blotting). This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.  Recommended Working Dilutions: Histochemical and cytochemical: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/6,000.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly purified lysozyme is isolated from pooled milk. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity	The reactivity of the antiserum is restricted to lysozyme. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against normal human milk and tears a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified lysozyme. No reaction is obtained with any other plasma protein component or serum. Cross-reactivity: The antiserum does not cross-react with any other component of human plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
Formulation	PBS, pH 7.2 Label: HRP State: Lyophilized Purified hyperimmune IgG fraction Stabilizer: None Preservative: None Label: Horseradish peroxidase enriched for isoenzyme C (RZ=3.2) Molar radio: ~ 1.7
Reconstitution Method	Restore by by adding 1 ml sterile di stilled water
Concentration	lot specific
Purification	Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt precipitation and purification of the IgG fraction by DEAE chromatography
Conjugation	HRP
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month  or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	lysozyme (renal amyloidosis)
Database Link	Entrez Gene 396218 Chicken UniProt ID P00698
Background	Lysozyme, a bacteriolytic enzyme, has a molecular weight of about 15,000. Its concentration in plasma is low; in milk the concentration is reported to be between 5 and 220 mg/ml. Also tears contain a considerable amount of lysozyme.
Synonyms	LYZ, LZM
Note	Conjugation procedure: Conjugation is carried out using a proprietary modification of the periodate technique for the binding to peroxidase, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilized to assure stability and long shelf life.
Reference Data

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