HRH2 (C-term) Goat Polyclonal Antibody

Specifications

Product Data
Applications	FC, IF, PEP-ELISA, WB
Recommend Dilution	Peptide ELISA: 1/128000 (Detection Limit).  Western blot: Preliminary experiments gave bands at approx 55kDa and a stronger 28kDa in Human Colon and Duodenum lysates after 0.1 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 40.1kDa according to NP_071640.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). Immunohistochemistry/Immunofluorescence: This antibody was successfully used on coronal Rat brain slices as reported in Peng et al, Neurosci Lett. 2013 Apr 29;541:87-92. PMID:23466693.
Reactivity	Canine, Human, Mouse, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the C-Terminus of the protein sequence according to NP_071640.1; NP_001124527.1.
Specificity	This antibody is expected to recognise both reported isoforms.
Formulation	Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified Ig fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	histamine receptor H2
Database Link	Entrez Gene 3274 Human UniProt ID P25021
Synonyms	H2R, HRH2, Gastric receptor I
Reference Data

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