AMPK alpha 2 (PRKAA2) (Center)/(Thr172) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, IHC, WB
Recommend Dilution	Western Blot:  1/1000 (tested in K562 cell line lysates (35µg/lane). Immunohistochemistry on paraffin sections: 1/10 - 1/50. Flow Cytometry: 1/10 - 1/50. ELISA: 1/1000.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	KLH conjugated synthetic peptide between 145-173 amino acids from the Central region of human PRKAA2 (Thr172)
Specificity	This antibody reacts to PRKAA2.
Formulation	PBS State: Aff - Purified State: Liquid purified Ig fraction Preservative: 0.09% (W/V) sodium azide
Concentration	lot specific
Purification	Affinity chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	62320 Da
Gene Name	protein kinase AMP-activated catalytic subunit alpha 2
Database Link	Entrez Gene 5563 Human UniProt ID P54646
Background	The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.
Synonyms	AMPK2, AMPK alpha-2 chain
Reference Data
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	Adipocytokine signaling pathway, Hypertrophic cardiomyopathy (HCM), Insulin signaling pathway, mTOR signaling pathway, Regulation of autophagy

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