Plakophilin 3 (PKP3) Mouse Monoclonal Antibody [Clone ID: 23E3/4]

Specifications

Product Data
Clone Name	23E3/4
Applications	IF, IHC, WB
Recommend Dilution	Western blot (Detection).  Immunoprecipitation  Immunocytochemistry (Methanol-and paraformaldehyde-fixed cells).  Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Embedded Sections. For optimal results, fixed cells and frozen tissues should be treated with 0.2% Triton-X 100 for, respectively, 5 min and 15 min prior to incubation with primary antibody. Recommended Dilutions:  1/25-1/200 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent and 1/100-1/1000 for Immunoblotting.
Reactivity	Canine, Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide corresponding to amino acid residues 570-589 of Human Plakophilin-3 coupled to Keyhole Limpet Hemocyanin via an additional cysteine residue at the N-terminus (CFTPQSRRLRELPLAADALTF).
Specificity	This antibody 23E3/4 reacts with an epitope located between residues 570-589 in Human Plakophilin-3.
Formulation	PBS with 0.09% Sodium Azide as preservative State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles.
Gene Name	plakophilin 3
Database Link	Entrez Gene 11187 Human UniProt ID Q9Y446
Background	Plakophilin-3 is an Armadillo-like protein present in nuclei and desmosomes of epithelial cells [1]. The expression pattern of this protein seems to be largely restricted to epithelial cell types. Plakophilin- 3 can be detected along cell borders in a punctuate staining pattern typical for desmosomal proteins. In addition to the desmosomal immunolocalisation, immunostaining was observed as bright nuclear speckles. Thus, like plakophilin-1 and-2, plakophilin- 3 displays a dual intracellular localisation in the desmosomal plaque and in the cell nucleus, and therefore is probably involved in signal transduction pathways between the plasma membrane and the nucleus. The human protein has a predicted molecular mass of 87 kD.
Synonyms	Plakophilin-3
Reference Data

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