Transferrin (N-term) Mouse Monoclonal Antibody [Clone ID: HTF-14]

CAT#: BM745AF

Transferrin (N-term) mouse monoclonal antibody, clone HTF-14, Azide Free



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CNY 3,140.00


货期*
5周

规格
    • 100 ug

Product images

Specifications

Product Data
Clone Name HTF-14
Applications ELISA, FN, IF, IHC, R, WB
Recommend Dilution Western blot: non-reducing conditions.
Immunohistochemistry on paraffin sections: 10 µg/ml.
Positive control: placenta.
Immunocytochemistry.
ELISA.
RIA.
Functional application: blocks binding of transferrin to the receptor.
Reactivity Human, Porcine, Rabbit
Host Mouse
Clonality Monoclonal
Immunogen Purified porcine transferrin
Specificity This antibody recognizes an epitope located in the N-terminal domain of human serum transferrin, a 77 kDa single polypeptide chain glycoprotein (member of the iron binding family of proteins). It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+.
Formulation Phosphate buffered saline (PBS), approx. pH 7.4; 0.2 μm filter sterilized
State: Azide Free
State: Liquid purified Ig fraction
Concentration lot specific
Purification Purified by protein-A affinity chromatography.
Conjugation Unconjugated
Storage Condition Store undiluted at 2-8°C.
DO NOT FREEZE!
Background Transferrin is a monomeric glycoprotein of approximately 77 kDa, which serves as an iron-transporter. In normal plasma, transferrin has a concentration of 25-50 µmol / liter, and is usually about one-third saturated with iron, thus providing a large buffering capacity in case of an acute increase in plasma iron levels. Cells take up transferrin-iron complexes (holotransferrin) using transferrin receptor dimers. Upon binding of holotransferrin, the receptor is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with its receptor (CD71) and recycles to the cell surface, where apotransferrin is released upon exposure to normal pH. Internalization of labeled transferrin thus represents an usefull approach to study endocytosis. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. Iron/transferrin complex is essential in haemoglobin synthesis and for certain types of cell division.
Synonyms Serotransferrin, Siderophilin
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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