MRP8/14 (S100A8/A9) Chicken Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC
Recommend Dilution	ELISA. Immunohistochemistry on Frozen Sections: 1 μg/ml (1/1000). Immunohistochemistry on Paraffin Sections: 4 μg/ml (1/250). Proteinase K pretreatment for antigen retrieval is recommended. Suggested Positive Control:Human tonsil.
Reactivity	Bovine, Human, Porcine
Host	Chicken
Clonality	Polyclonal
Immunogen	Recombinant Human MRP8 and MRP14.  Antigen, epitope: MRP8 and MRP14 and their Ca2+-dependent complex. Several epitopes.
Specificity	This polyclonal antibody recognizes different epitopes on the MRP8/14 heterocomplex (S100A8/S100A9). Thus, the antibody does not discriminate between the individual subunits MRP8 or MRP14. In immune histochemistry, it detects early inflammatory macrophages and can thus be used for the classification of acute stage inflammation in tissue sections and in smears. The MRP8/14 specific ELISA has shown significant advantages in the early assessment of certain acute inflammatory conditions.
Formulation	PBS, pH 7.2 Label: Biotin State: Liquid purified Ig fraction Preservative: Kathon
Concentration	1.0 mg/ml
Purification	Purified from egg yolk by repeated precipitation
Conjugation	Biotin
Storage Condition	Store the antibody undiluted at 2-8 °C.
Background	MRP8 and MRP14 are members of the S100 family of proteins containing 2 EF hand calcium binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21
Synonyms	Calgranulin A/B, Calprotectin, L1 Protein, CFAG, P8, P14, CAGA, CAGB, CFAG, MRP-8, MRP-14
Reference Data

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