Collagen I (COL1A1) Rabbit Polyclonal Antibody

CAT#: BP8028S

Rabbit Polyclonal Anti-COL1A1 Antibody, Purified



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BP8028S is a replacement of BP8028.

CNY 7,313.00


货期*
5周

规格
    • 100 ul

Product images

Specifications

Product Data
Applications ELISA, IF, IHC, R, WB
Recommend Dilution RIA.
ELISA: 1/200 (OD ≥ 500).
Immunflourescence Assays: 1/80.
Immunohistochemistry on Paraffin Sections: 1/200-1/600.
Pretreatment: After de-waxing the tissue slices they are treated with 0.2% Hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
Incubation Time: 60 min at RT or 2-8°C over night.
Positive Control: Human skin.
Reactivity Human
Host Rabbit
Clonality Polyclonal
Immunogen Purified Collagen type I from Human skin.
Specificity Human Collagen type I: 100%
Collagen type II and IV: < 0.1%
Collagen type III and V: < 1.0%
Human Fibronectin: < 0.1%
Cross-reactivity with cattle <1.0%, rat and chicken < 0.1% (RIA at a 1:500 dilution)
Formulation Phosphate buffered solution, no BSA and preservative added.
State: Purified
State: Lyophilized purified Ig fraction
Reconstitution Method Restore with 0.1 ml distilled water.
Concentration ~1.0 mg/ml (after reconstitution)
Purification Affinity Chromatography
Conjugation Unconjugated
Storage Condition Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Predicted Protein Size 136.5 kDa
Gene Name collagen type I alpha 1
Background Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs.
Type I collagen (95 kDa) is found in bone, cornea, skin and tendon.
Synonyms OI4
Reference Data
Protein Families Druggable Genome
Protein Pathways ECM-receptor interaction, Focal adhesion
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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