Cd8a Rat Monoclonal Antibody [Clone ID: YTS169.4]
CAT#: CL007F
Cd8a rat monoclonal antibody, clone YTS169.4, FITC
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CNY 3,740.00
货期*
5周
规格
Specifications
Product Data | |
Clone Name | YTS169.4 |
Applications | FC, IHC |
Recommend Dilution | Flow Cytometry. Immunohistochemistry on frozen sections. |
Reactivity | Mouse |
Host | Rat |
Clonality | Monoclonal |
Immunogen | Murine thymocytes |
Specificity | Antibody CL007 reacts with a protein of approximately 30 kDa found on mouse thymocytes and mouse cytotoxic/ suppressor T cells. It does not bind to mouse helper/inducer T cells. It binds to T lymphocytes from all mouse strains regardless of phenotypic expression (i.e. reacts with T lymphocytes from mouse strains expressing the Ly 2.1 or Ly 2.2 phenotype). It can be used to investigate the role of T cells in models for infectious disease, autoimmunity, transplantation tolerance and fundamental aspects of immunology. |
Formulation | PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. Label: FITC State: Liquid purified IgG |
Concentration | lot specific |
Purification | Protein G Chromatography |
Conjugation | FITC |
Storage Condition | Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. |
Gene Name | CD8 antigen, alpha chain |
Database Link | |
Background | The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. |
Synonyms | CD8 alpha chain, CD8A, MAL |
Note | Strain Distribution by Flow Cytometry Analysis: Procedure: see below Cell Concentration: 1x10e6 cells per test Antibody Concentration Used: 0.1 µg/10e6 cells Strains Tested: BALB/c, C57BL/6 Positive: BALB/c, C57BL/6 Negative: non Protocol: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x107 cells/ml in media A. Add 50µl of this suspension to each tube (each tube will then contain 1 x 106 cells, representing 1 test). 4. To each tube, add 0.1-0.5 µg* of CL007F per 106 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes are protected from light, since most flurochromes are light sensitive.) 7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 µl of 2M sodium azide in 100 mls). |
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