GM CSF (CSF2) Mouse Monoclonal Antibody [Clone ID: 59]

Specifications

Product Data
Clone Name	59
Applications	ELISA, WB
Recommend Dilution	ELISA: This antibody can be used as a Capture antibody in Sandwich ELISA applications for Human GM-CSF detection in combination with a monoclonal tracer/detection antibody (HRP conjugated Cat.-No DM1011HRP or Biotin conjugated Cat.-No DM1011BT). Suggested Capture Coating Dose: 0.3 µg/well. Substrate: TMB. If the above suggested conditions are followed approximately 2 pg/mL of GM-CSF in serum/plasma or medium can be detected with an assay range of 0-500 pg/ml.  Western Blot.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant human GM-CSF Mouse IgG1.
Specificity	This monoclonal antibody reacts with natural and recombinant Human GM-CSF. Does not show any cross reaction with other Human Cytokines or Growth Factors tested such as M-CSF, G-CSFR, IL-8, IL-16, IL1-beta, TGF beta-1 and TNF-alpha.
Formulation	0.01M PBS, pH 7.2 without preservatives. State: Purified State: Lyophilized purified IgG fraction.
Reconstitution Method	Restore with double distillated water to adjust the final concentration to 1.00 mg/ml
Concentration	1.0 mg/ml (after reconstitution)
Purification	Affinity Chromatography on Protein G.
Conjugation	Unconjugated
Storage Condition	Upon receipt, store (in aliquots) at -20°C.  Avoid repeated freezing and thawing.
Gene Name	colony stimulating factor 2
Database Link	Entrez Gene 1437 Human UniProt ID P04141
Background	The cytokine GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Used in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia.
Synonyms	CSF2, GMCSF, Sargramostim, Molgramostin
Reference Data

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