CEACAM5 Mouse Monoclonal Antibody [Clone ID: D14HD11]

Specifications

Product Data
Clone Name	D14HD11
Applications	ELISA, FC, IF, IHC, WB
Recommend Dilution	Flow Cytometry: 1.2 µg/106 cells. The antibody is routinely tested on BOSC23 cells transiently transfected with a CEACAM5 expression vector. Cell based ELISA with intakt, transiently transfected cells: 1/200. ELISA: 1/200-1/400. Western blot: 4 µg/ml. Immunofluorescence: 1 µg/106 cells. For various aplications see list of references.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Immunisation with extracted protein of CEACAM5
Specificity	D14HD11 is a broadly reactive antibody which reacts with high affinity (affinity to solid phase bound CEACAM5: 2 x 10e10 L/Mol [6]) with most of the CEACAM molecules (except CEACAM7 and CEACAM8) transiently expressed on the cell surface of transfected BOSC23 cells. D14HD11 was included and characterized in the studies from the VIth Leucocyte Typing Workshop. It reacts with the CEACAM family N-domain.
Formulation	PBS, pH 7.2 State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	carcinoembryonic antigen related cell adhesion molecule 5
Database Link	Entrez Gene 1048 Human UniProt ID P06731
Background	CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family (1). The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains (1,2). CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosylphosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/CEACAM5 in tumours of epithelial origin is the basis of its wide-spread use as a tumour marker (2). The function of CEACAM family members varies widely: they function as cell adhesion molecules, tumour suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria (1).
Synonyms	CEA, Carcinoembryonic antigen
Reference Data

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