Leptin (LEP) Mouse Monoclonal Antibody [Clone ID: LEP-02]

Specifications

Product Data
Clone Name	LEP-02
Applications	ELISA, WB
Recommend Dilution	ELISA. Western Blot.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	The Human Leptin is a recombinant protein produced in E.coli.
Specificity	The Anti Human Leptin Antibody, Clone LEP-02 is a Mouse monoclonal antibody against Human recombinant Human Leptin.  It does not react with Mouse.
Formulation	0.05M phosphate buffer, 0.1M NaCl, pH 7.2. Azide Free State: Aff - Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore with 0.1 ml of deionized water
Concentration	1.0 mg/ml (after reconstitution)
Purification	Affinity Chromatography on Immobilized Protein G
Conjugation	Unconjugated
Storage Condition	Store lyophilized (preferably in a desiccator) at -20°C and in aliquots at -80°C. Reconstituted antibody can be stored at 2-8°C for a limited period of time; it does not show decline in activity after two weeks at 4°C. Avoid repeated freezing and thawing.
Gene Name	leptin
Database Link	Entrez Gene 3952 Human UniProt ID P41159
Background	Leptin, the product of the ob (obese) gene, is a single-chain 16 kDa protein consisting of 146 amino acid residues. Leptin is produced mainly in the adipose tissue, and is considered to play an important role in appetite control, fat metabolism and body weight regulation. It targets the central nervous system, particularly hypothalamus, affecting food intake. The primary effect of leptin appears to be mediated by leptin receptors expressed mainly in the hypothalamus. In humans, leptin levels correlate with body mass index (BMI) and percentage body fat, and are elevated even in obese individuals. Leptin has a dual action; it decreases the appetite and increases energy consumption, causing more fat to be burned. Leptin is secreted in circadian fashion with nocturnal rise in both lean and obese patients. Mutations of the ob gene resulting in leptin deficiency are the cause of obesity in the ob/ob mice. Endogeneous leptin can normalize their body weight. In contrast, high levels of leptin in obese human subjects point to an insensitivity to endogeneous leptin. Other factors in addition to the amount of body fat appear to regulate leptin action: insulin, glucocorticoids, catecholamines and sex hormones. Studies have shown that leptin may be linked to reproductive function.
Synonyms	LEP, OB, OBS, Obesity factor, Obese protein
Reference Data

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