VEGF Receptor 1 (FLT1) Mouse Monoclonal Antibody [Clone ID: #EWC]

Specifications

Product Data
Clone Name	#EWC
Applications	ELISA, IF, WB
Recommend Dilution	ELISA: 1-10 µg/ml IgG1. Western Blot: 2-5 µg/ml IgG1. Immunofluorescence: 2-10 µg/ml.
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Human soluble extracellular Flt-1 protein (D5) (Cat.-No DA3539X)
Specificity	The monoclonal antibody will detect native and denaturated VEGFR-1/Flt-1 in ELISA experiments and on the surface of different Human cell types. The antibody will also detect Mouse Flt-1. This clone was formerly named FLTEWC.
Formulation	PBS, pH 6.0 without preservatives or stabilizers State: Purified State: Lyophilized purified IgG fraction from cell culture supernatant
Reconstitution Method	Restore with distilled sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Protein G Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	fms related tyrosine kinase 1
Database Link	Entrez Gene 2321 Human UniProt ID P17948
Background	VEGF Receptor 1 (also known as FLT) belongs to the src gene family and shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. The protein acts as a receptor for VEGF, VEGFB and PGF. An alternatively spliced form of the gene produces a soluble protein (sFlt1) which binds vascular endothelial growth factor (VEGF) with high affinity. sFlt1 has a higher affinity for VEGF indicating that it may function as an inhibitor in the VEGF response. VEGF Receptor 1 is specifically expressed in most vascular endothelial cells and peripheral blood monocytes. VEGF and its high-affinity binding receptors, the tyrosine kinases FLK1 and FLT1, are thought to be important for the development of embryonic vasculature. It has been shown that an alternately spliced form of FLT1 produces a soluble protein, termed sFLT1, which binds vascular endothelial growth factor with high affinity. Because sFLT1 has a higher affinity for VEGF than does FLK1, it may function as an inhibitor of VEGF response.
Synonyms	VEGFR1, FLT1, FLT, FRT, VEGF Receptor 1
Reference Data

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