Il4 Rabbit Polyclonal Antibody

CAT#: PP035B1

Il4 rabbit polyclonal antibody, Biotin

Conjugation: Unconjugated Biotin



Need it in bulk or conjugated?
Get a free quote

CNY 3,910.00


货期*
5周

规格
    • 25 ug

Product images

Specifications

Product Data
Applications ELISA, WB
Recommend Dilution ELISA:
To detect rat IL-4 by direct ELISA (using 100 ml/well antibody solution) this antibody can be used at a concentration of 0.15-0.30 µg/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant rat IL-4.
Western Blot:
To detect rat IL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat IL-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity Rat
Host Rabbit
Clonality Polyclonal
Immunogen Highly pure (>98%) recombinant rat IL-4 (rat Interleukin-4).
Specificity This antibody recognises Rat IL-4.
Formulation PBS, pH 7.2 without preservatives.
Label: Biotin
State: Lyophilized purified Ig fraction.
Label: conjugated
Reconstitution Method Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification Affinity chromatography.
Conjugation Biotin
Storage Condition Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer.
Avoid repeated freezing and thawing.
Gene Name interleukin 4
Background IL4 is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. It is a ligand for Interleukin 4 receptor. The Interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. IL4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of naïve CD4+ T cell to the Th2 type. Th2 cells produce IL4, IL5, IL10 and IL13, which tend to favor a humoral immune response while suppressing a cell mediated immune response controlled by Th1 cells. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. The second is regulating IgE and IgG1 production by B cells. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Synonyms IL-4, BSF1, Lymphocyte stimulatory factor 1, Binetrakin, Pitrakinra
Note Centrifuge vial prior to opening!
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
Customer Reviews 
Loading...