Cxcl12 (alpha) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Direct ELISA: To detect Mouse SDF-1 alpha by Direct ELISA (using 100 µl/well antibody solution) this antibody can be used at a concentration of ~1.0 µg/ml. This biotinylated polyclonal antibody allows the detection of at least 0.2-0.4 ng/well of recombinant Mouse SDF-1 alpha. Sandwich ELISA: To detect Mouse SDF-1 alpha by Sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.25–1.0 µg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Anti-Mouse SDF-1 alpha (Cat.-No PP1217P1 or PP1217P2) as a Capture antibody, allows the detection of at least 0.2–0.4 ng/well of recombinant Mouse SDF-1 alpha. Western Blot: To detect Mouse SDF-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Mouse SDF-1 alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Mouse
Host	Goat
Clonality	Polyclonal
Immunogen	Highly pure (>98%) E.coli derived recombinant Mouse SDF-1 alpha (Cat.-No PA152)
Specificity	Reacts with Mouse Stromal Cell Derived Factor-1 alpha (SDF-1 alpha). Other species not tested.
Formulation	PBS, pH 7.2 without preservatives Label: Biotin State: Lyophilized (sterile filtered) purified Ig fraction Label: conjugated
Reconstitution Method	Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography
Conjugation	Biotin
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Predicted Protein Size	~150 kDa
Gene Name	chemokine (C-X-C motif) ligand 12
Database Link	Entrez Gene 20315 Mouse UniProt ID P40224
Background	SDF1 alpha and 1 beta are small cytokines that belong to the intercrine family, members of which activate leukocytes and are often induced by proinflammatory stimuli such as lipopolysaccharide, TNF or IL1. SDF1 alpha has been shown to be chemotactic for lymphocytes and is recently reported to be a ligand for CXCR4 (LESTR/fusin), a co-receptor for HIV1 entry into T cells. SDF-1 binding to CXCR4 inhibits HIV1 entry.
Synonyms	Stromal cell-derived factor 1, SDF1A, SDF1B, IRH, hIRH, PBSF
Reference Data

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