L-ascorbate oxidase Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Western blot: 1/500-1/5000. Immunoprecipitation: 1/100. ELISA: 1/5000-1/20000.
Reactivity	Rabbit, Cucurbita
Host	Rabbit
Clonality	Polyclonal
Immunogen	Ascorbate oxidase  from cucurbita
Specificity	This antibody detects ascorbate oxidase [cucurbita]. Cross reactivity against ascorbate oxidase from other sources is unknown. Immunoelectrophoresis gives a single precipitin arc against anti-peroxidase, anti-rabbit serum and purified and partially purified ascorbate oxidase [cucurbita].
Formulation	0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: HRP State: Lyophilized purified Ig fraction. Preservative: 0.09% Gentamicin sulfate (Do NOT add Sodium azide!) Label: Horseradish peroxidase
Reconstitution Method	Restore with 0.1 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation	HRP
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Database Link	UniProt ID P24792
Synonyms	AAO
Reference Data

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