CELA1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Suitable for immunoblotting (Western or dot blot), ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity. Recommended Dilutions: Western blot: 1/500-1/3,000. ELISA: 1/5,000-1/25,000 This product has been assayed against 1.0 µg of elastase [porcine pancreas] in a standard sandwich ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested.
Reactivity	Porcine
Host	Rabbit
Clonality	Polyclonal
Immunogen	Elastase from porcine pancreas
Specificity	This antibody detects porcine elastase. Cross reactivity against elastase from other tissues and species has not been determined. Immunoelectrophoresis gives a single precipitin arc against anti-biotin, anti-rabbit serum as well as purified and partially purified elastase [porcine pancreas].
Formulation	0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: Biotin State: Purified State: Lyophilized purified Ig fraction Stabilizer: 10 mg/ml BSA (immunoglobulin and protease) Preservative: 0.01% Sodium azide
Reconstitution Method	Restore with 0.1 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation	Biotin
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Database Link	Entrez Gene 396766 Pig UniProt ID P00772
Background	Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Elastase is a serine protease which exhibits endopeptidase activity on many proteins, its principal substrate, however, is elastin and it is unique among proteases in its ability to hydrolyse native elastin. It is present in the pancreas, leukocytes and serum. Elastase consists of a single polypeptide chain with a molecular weight of 29.5kDa. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Like most of the human elastases, elastase 2A is secreted from the pancreas as a zymogen. In other species, elastase 2A has been shown to preferentially cleave proteins after leucine, methionine, and phenylalanine residues. Elastase 1 is not a pancreatic enzyme: clinical literature that describes human elastase 1 activity in the pancreas is actually referring to elastase 2A.
Synonyms	ELA1, CELA1
Reference Data

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