LDHA Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IP, WB
Recommend Dilution	Suitable for use in ELISA (1/4,000-1/20,000) and Western blot (1/500-1/2,000), Immunoprecipitation (1/100). Expect a band approximately 36 kDa in size corresponding to LDH western blotting in the appropriate cell lysate or extract.
Reactivity	Rabbit
Host	Goat
Clonality	Polyclonal
Immunogen	Full length lactate dehydrogenase protein isolated from Rabbit muscle.
Specificity	Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Lactate Dehydrogenase [Rabbit Muscle]. BLAST analysis was used to determine that cross reactivity is suggested for both muscle and heart isoforms (LDH-A and LDH-B) from most mammalian species.
Formulation	0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 State: Serum State: Lyophilized purified Ig fraction Stabilizer: None Preservative: 0.01% Sodium Azide
Reconstitution Method	Restore with 2.0 ml of deionized water (or equivalent)
Concentration	lot specific
Purification	Prepared from monospecific antiserum by delipidation and defibrination
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	Entrez Gene 100009107 Rabbit UniProt ID P13491
Background	Lactate dehydrogenase is also known as L-lactate dehydrogenase A chain, LDH-A, LDH muscle subunit and LDH-M. Two isozymes of LDH occur in mammals, LDH-M and LDH-H which come together to form a homotetramer of 36 kDa subunits. Every LDH molecule consists of four subunits, where each subunit is either H each M (based on their electrophoretic properties.) There are, therefore, five LDH isotypes: LDH-1 (4H) - in the heart, LDH-2 (3H1M) - in the reticuloendothelial system, LDH-3 (2H2M) - in the lungs, LDH-4 (1H3M) - in the kidneys and LDH-5 (4M) - in the liver and striated muscle. Usually LDH-2 is the predominant form in the serum. An LDH-1 level higher than the LDH-2 level (a "flipped pattern") suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH-1, into the bloodstream). In general, LDH is often used as a marker of tissue breakdown. LDH shows a cytoplasmic localization.
Synonyms	LDH-A, L-lactate dehydrogenase A chain, LDH-M, PIG19, NY-REN-59
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.