pep1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	Western Blot: 1/1,000-1/5,000. ELISA: 1/14,000. This product has been assayed against 1.0 µg of Phospho-enol-pyruvate carboxylase [maize] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature.  A working dilution of 1/1,000 to 1/6,000 of the reconstitution concentration is suggested. Immunohistochemistry: Paraffin (FFPE) or Frozen sections; 1/100 suggested to begin.
Reactivity	Maize
Host	Rabbit
Clonality	Polyclonal
Immunogen	Phospho-enol-pyruvate carboxylase / PEP1 from maize
Specificity	This antibody detects PEP1 [maize leaves]. Cross reactivity against Phospho-enol-pyruvate carboxylase from other tissues and species may occur but have not been specifically determined. Immunoelectrophoresis give a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as purified and partially purified Phospho-enol-pyruvate carboxylase [maize].
Formulation	0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: HRP State: Purified State: Lyophilized purified Ig fraction Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free) Preservative: 0.01% Gentamicin sulfate (Do NOT add Sodium azide!) Label: Horseradish peroxidase
Reconstitution Method	Restore with 0.1 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation	HRP
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Database Link	UniProt ID P04711
Synonyms	PPC, PEPC1, PEPC 1, PEPCase 1, PEP carboxylase
Reference Data

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