CD4 Mouse Monoclonal Antibody [Clone ID: RPA-T4]

Specifications

Product Data
Clone Name	RPA-T4
Applications	FC, IHC
Recommend Dilution	Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 10e6 cells or 100 µl whole blood. Immunohistochemistry on Frozen Sections: 1/10-1/100. Recommended Positive Control: Human lymphatic tissue.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human PHA blasts. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Specificity	This antibody recognises CD4. Epitope mapping studies have shown that antibodies, produced by clone RPA-T4, recognise an epitope within domain 1, of the extracellular region, of the CD4 molecule. Clone RPA-T4 has been reported to block gp120-CD4 interaction and inhibit syncytium formation. We recommend the use of SM1058LE for this purpose.
Formulation	PBS, pH 7.4 containing 0.09% Sodium Azide as preservative State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD4 molecule
Database Link	Entrez Gene 920 Human UniProt ID P01730
Background	CD4 is a 55kD cell surface glycoprotein that is primarily expressed on a subpopulation of T lymphocytes, on peripheral blood monocytes and on tissue macrophages.
Synonyms	T-cell surface antigen T4/Leu-3
Reference Data

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