Aggrecan (ACAN) Mouse Monoclonal Antibody [Clone ID: HAG7D4 (7D4)]

Specifications

Product Data
Clone Name	HAG7D4 (7D4)
Applications	ELISA, IHC, IP, WB
Recommend Dilution	ELISA. Western blot. Immunoprecipitation. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: Requires a standard heat mediated antigen retrieval using a microwave. Histology Positive Control Tissue: Cerebral Cortex.
Reactivity	Bovine, Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified Human articular cartilage Aggrecan.
Specificity	This antibody Clone 7D4 Cat.-No SM1353P recognizes Human Aggrecan, a proteoglycan component of cartilage matrix and member of the aggrecan/versican proteoglycan family, which forms a major component of the extracellular matrix (ECM) of both cartilage and the central nervous system (CNS). Studies also showed a difference in the relative abundance of these isoforms when comparing brain and cartilage tissues (Virgintino et al. 2009 in Ref.1).  SM1353P recognizes epitopes within the N-terminal G1-IGD-G2 region of Aggrecan, that has been identified as a cluster 1 isoform.  Negative Species: Chicken, Fish and Rat.
Formulation	PBS State: Purified State: Liquid purified IgG fraction from Tissue Culture Supernatant Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	aggrecan
Database Link	Entrez Gene 176 Human UniProt ID P16112
Background	Aggrecan is a member of a family of large, aggregating proteoglycans (also including versican, brevican and neurocan) which is found in articular cartilage. Aggrecan is composed of three major domains: G1, G2, and G3. Between the G1 and G2 domains there is an interglobulin region (IGD). The IGD region is the major site of cleavage by specific proteases like metalloproteinases (MMPs) and aggrecanase. Aggrecan cleavage has been associated with a number of degenerative diseases including rheumatoid arthritis and osteoarthritis. There is evidence that this family of proteoglycans modulates cell adhesion, migration, and axonal outgrowth in the CNS.
Synonyms	ACAN, AGC1, CSPG1, MSK16
Reference Data

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