Pk (V5) Epitope Tag (GKPIPNPLLGLDST) Mouse Monoclonal Antibody [Clone ID: SV5-PK1]
CAT#: SM1691B
Pk (V5) Epitope Tag (GKPIPNPLLGLDST) mouse monoclonal antibody, clone SV5-PK1, Biotin
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CNY 5,731.00
货期*
4周
规格
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Specifications
Product Data | |
Clone Name | SV5-PK1 |
Applications | ELISA, IHC, WB |
Recommend Dilution | ELISA. Western Blot: 1/1000-1/5000. Immunohistochemistry on Frozen Sections. |
Host | Mouse |
Clonality | Monoclonal |
Immunogen | Mice were infected with the paramyxovirus SV5, Simian-Virus 5. Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 Ag14 myeloma cell line. |
Specificity | This antibody recognises a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. This antibody has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro ile pro asn pro leu leu gly leu asp ser thr. (the 9 amino acid epitope is underlined) |
Formulation | PBS containing 0.09% Sodium Azide as preservative Label: Biotin State: Liquid purified IgG fraction |
Concentration | lot specific |
Purification | Affinity Chromatography on Protein G |
Conjugation | Biotin |
Storage Condition | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
Note | This product is manufactured under an exclusive license from the University of St. Andrews,UK. |
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