IL4 Mouse Monoclonal Antibody [Clone ID: CC313]

Specifications

Product Data
Clone Name	CC313
Applications	ELISA, FC
Recommend Dilution	Flow Cytometry. ELISA: 2-5 µg/ml.  This reagent may be used as a capture antibody in ELISA assays for Bovine IL-4 in combination with SM2242B as a detection reagent, and recombinant Bovine IL-4 (SA1007) as a standard.
Reactivity	Bovine, Goat, Sheep
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Bovine IL-4. Spleen cells form immunised BALB/c mice were fused with cells of the SP2/O myeloma cell line.
Specificity	This antibody is specific for Interleukin 4 (IL-4). This Mouse anti Bovine interleukin-4, clone CC313 is specific for Bovine Interleukin 4 (IL-4), also known as B-cell stimulatory factor 1. Mouse anti Bovine interleukin-4, clone CC313 has been used successfully as a coating antibody in a sandwich ELISA with Clone CC314 as a detection reagent both with bovine (Stabel et al. 2011) and ovine samples (Olivier et al. 2012). Clones CC313 and CC314 have been used in the reverse configuration for the measurement of interleukin-4 in caprine samples (Marinaro et al. 2012).
Formulation	PBS, pH 7.4 State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	Entrez Gene 280824 Bovine UniProt ID P30367
Background	Interleukin 4 (IL-4), also known as B-cell stimulatory factor 1. Bovine interleukin-4 is a 15kDa, 135 amino acid peptide which is processed to a mature secreted form with the cleavage of a 24 amino acid, N-terminal signal peptide resulting in a 12.6kDa active cytokine, the apparent Mw may be altered by glycosylation (Heussler et al. 1992).  IL-4 is expressed by a range of cells including T and B cells, macrophages and monocytes. It plays an important role in the regulation of bovine T and B cell responses.
Synonyms	IL-4, BSF1, Lymphocyte stimulatory factor 1, Binetrakin, Pitrakinra
Reference Data

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