MCP1 (CCL2) Mouse Monoclonal Antibody [Clone ID: 2.2-4A4-1A11]

Specifications

Product Data
Clone Name	2.2-4A4-1A11
Applications	ELISA, IHC
Recommend Dilution	ELISA: 1-2 µg/ml.  This product may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA as a detection reagent. Western Blot: 0.2-0.4 µg/ml.  Immunohistochemistry on paraffin sections: 10 µg/ml. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant human MCP-1/MCAF (Macrophage Chemoattractant Activating Factor). Spleen cells from immunised BALB/c X ICR F1 mice were fused with cells of a mouse myeloma cell line.
Specificity	This antibody specifically recognises MCP-1, also known as CCL2, and does not cross react with MCP-2, MCP-3 or other structurally related chemokines. This antibody, clone 2.2-4A4-1A11 recognizes both native and recombinant MCP1 in a sensitive sandwich ELISA assay.
Formulation	PBS, pH7.2 State: Purified State: Lyophilized purified IgG
Reconstitution Method	Restore with 0.5 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. We recommend that the vial is gently mixed after reconstitution.
Concentration	lot specific
Purification	Affinity chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	C-C motif chemokine ligand 2
Database Link	Entrez Gene 6347 Human UniProt ID P13500
Background	This chemotactic factor attracts monocytes and basophils but not neutrophils or eosinophils and augments monocyte anti-tumor activity. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. It may be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
Synonyms	C-C motif chemokine 2, SCYA2, MCAF, Small-inducible cytokine A2, MCP-1, HC11, HC-11
Reference Data

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