Cd8a Mouse Monoclonal Antibody [Clone ID: OX-8]

Specifications

Product Data
Clone Name	OX-8
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	Flow Cytometry: 1/100; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: This product does not require protein digestion pre-treatment of paraffin embedded sections; This product does not require antigen retrieval using heat treatment prior to staining of paraffin embedded sections.  Western blot. Immunofluorescence. Immunoprecipitation.
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Rat thymocyte membrane glycoproteins. Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
Specificity	This antibody Mouse anti Rat CD8α, clone MRC OX-8, recognizes the rat CD8 alpha cell surface antigen, expressed by a subset of T lymphocytes, most thymocytes and the majority of NK cells. Clone MRC OX-8 is suitable for use in in vitro blocking studies (Popov et al.2001). Mouse anti Rat CD8α, clone MRC OX-8 has been described reacting with paraffin-embedded material following PLP Fixation (periodate-lysine paraformaldehyde) (Whiteland et al. 1995). Mouse anti Rat CD8α, clone MRC OX-8 is routinely tested in flow cytometry on rat splenocytes.
Formulation	PBS, pH 7.2 containing 0.09% Sodium Azide State: Purified State: Liquid purified IgG
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD8a molecule
Database Link	Entrez Gene 24930 Rat UniProt ID P07725
Background	The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.
Synonyms	CD8 alpha chain, CD8A, MAL
Reference Data

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