CD59 Mouse Monoclonal Antibody [Clone ID: MEM-43/5]

Specifications

Product Data
Clone Name	MEM-43/5
Applications	FC, IHC, IP, WB
Recommend Dilution	Flow cytometry: Recommended dilution: 0.5-4 μg/ml; positive control: blood. Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/ml. Western blotting: Recommended dilution: 1-2 μg/ml; positive control: mouse spleen, non-reducing conditions.
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Thymocytes and T lymphocytes
Specificity	The antibody reacts with well defined epitope (around L33) on CD59 (Protectin), a 18-20 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on all hematopoietic cells; it is widely present on cells in all tissues. The antibody does not compete with most other CD59 antibodies.
Formulation	Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 State: Purified State: Liquid Ig fraction
Concentration	lot specific
Purification	Protein-A affinity chromatography; purity: > 95% (by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Gene Name	CD59 molecule
Database Link	Entrez Gene 966 Human UniProt ID P13987
Background	CD59 (Protectin) is a small (18-20 kDa) GPI-anchored ubiquitously expressed inhibitor of the membrane attack complex (MAC). It is thus the key regulator that preserves the autologous cells from terminal effector mechanism of the complement cascade. CD59 associates with C5b-8 complex and thereby counteracts appropriate formation of cytolytic pore within the plasma membrane. CD59 is also an low-affinity ligand of human CD2 and causes T cell costimulation.
Synonyms	MAC-inhibitory protein, Protectin, MEM43 antigen, MIC11, MIN1, MIN2, MIN3, MSK21, MACIF, MAC-IP, MIRL, HRF20, HRF-20
Reference Data

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