IFNG Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FN, WB
Recommend Dilution	ELISA.  Functional Assays: 10-50 µg/ml. Western Blot: This antibody detects a band of approximately 18 kDa.
Reactivity	Chicken
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant Chicken Interferon Gamma
Specificity	This antibody recognizes Chicken Interferon gamma (chIFN-gamma). This antibody can be used to neutralize chIFN-gamma activity and to discriminate the antiviral activity of chIFNg-amma from that of chIFN-alpha and chIFN-beta.
Formulation	PBS State: Azide Free State: Liquid purified IgG fraction Stabilizer: None Preservative: None
Concentration	lot specific
Purification	Affinity Chromatography on protein G
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	interferon, gamma
Database Link	Entrez Gene 396054 Chicken UniProt ID P49708
Background	Original studies investigating the properties of chicken Interferon (chIFN), revealed properties similar to mammalian IFN type I (IFNI), and it has since become apparent that there are at least three distinct chicken interferon proteins, designated as chIFN-alpha, chIFN-beta and chIFN-gamma. chIFN-gamma was first identified, when supernatant from chicken spleen cells stimulated with concanavalin A (ConA ), displayed macrophage-activating factor (MAF) activity - the principle function of mammalian type II IFNgamma. Several independent studies have since supported the existence of this chicken homolog, by the cloning of IFN-gamma cDNA from activated chicken T cells and demonstrating that recombinant chicken IFNgamma strongly upregulates the expression of MHC class II and iNOS genes.
Synonyms	IFN-gamma, gamma IFN
Reference Data

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