DDX58 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	RIG-1 antibody can be used for the detection of RIG-1 by Western blot at 0.5 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human and rat samples. All other applications and species not yet tested.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	RIG-1 antibody was raised against human GST-tagged RIG-1 protein.
Formulation	PBS containing 0.02% sodium azide.
Concentration	1 mg/ml
Purification	RIG-1 Antibody is Protein A purified.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	DEXD/H-box helicase 58
Database Link	NP_055129 Entrez Gene 230073 MouseEntrez Gene 297989 RatEntrez Gene 23586 Human UniProt ID O95786
Background	The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses. Like the toll-like receptor 3 (TLR3), the cytoplasmic helicase retinoic acid inducible gene protein 1 (RIG-1) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. Unlike TLR3 however, RIG-1 activates the kinases TBK1 and IKKepsilon through the adaptor protein IPS-1. These kinases then phosphorylate the transcription factors IRF-3 and IRF-7 which are essential for the expression of type-I interferons. RIG-1 is required for the production of interferons in response to RNA viruses including paramyxoviruses, influenza virus, and Japanese encephalitis virus.
Synonyms	RIG-I; RIGI; RLR-1
Reference Data
Protein Pathways	Cytosolic DNA-sensing pathway, RIG-I-like receptor signaling pathway

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